This is how we do it in our lab. We then calculate T2. First, update your CCPNMR if you are using new TopSpin. Bruker changed their format in TopSpin 3.5 and there is an update which fixes the problem that that caused. If you have any errors in ccpn do not hesitate to send it with your email address, Wayne is kindly responding for that. Open your pseudo 3D spectra. For T1 point values use vd list (seconds), for T1 rho vp list (in microseconds), for hetNOE 0,1. Somehow I had order problem before update: values for T1 started at second value and finished at first in ccpn order. So instead of 0.05, 1.2, 6, ..., 0.5, 1.8 I put: 1.2, 6, ..., 0.5, 1.8, 0.05. But after updated ccpn it was in right order. Molecule - Add Sequence Experiments - NMR series - add series Other - Format conventer - import - single files - peaks - sparky The right format should look like that: Assignment w1 w2 Q1N-HN 121.92 8.22 G2N-HN 102.15 5.58 Peak - peak list - copy p